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目的 研究 3 硝基丙酸 (3 nitropropionicacid ,3 NPA)多次预处理对多巴胺能神经元的保护机制。方法 应用MPTP在C5 7BL小鼠上制作帕金森病模型 ,应用爬杆、悬挂实验检测小鼠协调运动能力 ;应用免疫组化检测中脑黑质酪氨酸羟化酶 (TH)及神经凋亡抑制蛋白 (NAIP)的表达。结果 在MPTP组小鼠爬杆、悬挂实验评分降低 ,TH表达明显减少 ,无NAIP表达 ;3 硝基丙酸单次预处理后评分增加 ,TH、NAIP表达增多 ;多次预处理后TH、NAIP表达及评分增加更明显。结论 3 NPA多次预处理对多巴胺能神经元的保护机制与上调NAIP表达有关。
Objective To study the protective effect of 3-nitropropionic acid (3-NPA) on dopaminergic neurons. Methods MPTP was used to make Parkinson’s disease model in C5 7BL mice. The activity of coordination movement of mice was detected by climbing pole and suspension test. Tyrosine hydroxylase (TH) and neuronal apoptosis Inhibitory protein (NAIP) expression. Results In the MPTP group, the score of hanging test and hanging test decreased, the expression of TH decreased significantly, and NAIP was not expressed. The score of THP and NAIP increased after 3-nitropropionic acid pretreatment, while TH and NAIP Increased expression and score more pronounced. Conclusion 3 NPA multiple pretreatment of dopaminergic neurons protective mechanism and upregulation of NAIP expression.