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[目的]分析肺腺癌中染色体异常变化和基因改变,探讨肺腺癌细胞遗传物质的改变与肺腺癌发生发展之间的关系。[方法]应用M-FISH技术,检测3株肺腺癌细胞株的染色体数目及结构畸变情况,应用CGH技术对50例肺腺癌组织提取的全基因组DNA进行检测,以了解肺腺癌全基因组的变化。[结果]M-FISH结果显示肺腺癌细胞中存在许多复杂的染色体重排,5、6、11、12、17号染色体最频繁参与染色体间的易位。CGH发现,在50例肺腺癌标本基因组中,最常见的扩增区域是1q、2p、3q、5p、5q、7p、8q、11q、12q、14q、16p、17q、19q、20q、21q、22q。最常见的缺失区域是2q、3p、4p、5q、7q、8p、9p、13q、14q、17p。其中最常见的扩增位点是16p13,阳性率达50%,最常见的缺失位点是8p22,阳性率达38%。[结论]M-FISH和CGH技术是研究肺腺癌基因组变化的强有力工具,该实验中发现的基因可能代表了与肺腺癌的病理、诊断相关的候选基因。
[Objective] To analyze the chromosomal abnormalities and gene changes in lung adenocarcinoma and to explore the relationship between the changes of genetic material in lung adenocarcinoma cells and the occurrence and development of lung adenocarcinoma. [Methods] Chromosome number and structure aberration of three lung adenocarcinoma cell lines were detected by M-FISH. Genomic DNA extracted from 50 cases of lung adenocarcinoma was detected by CGH to understand the whole genome of lung adenocarcinoma The change. [Results] The results of M-FISH showed that there were many complex chromosomal rearrangements in lung adenocarcinoma cells. The chromosomes 5, 6, 11, 12 and 17 were the most frequently involved in inter-chromosomal translocation. CGH found that the most common amplification region in 50 lung adenocarcinoma specimens was 1q, 2p, 3q, 5p, 5q, 7p, 8q, 11q, 12q, 14q, 16p, 17q, 19q, 20q, 21q, 22q. The most common missing regions are 2q, 3p, 4p, 5q, 7q, 8p, 9p, 13q, 14q, 17p. One of the most common amplification sites is 16p13, the positive rate of 50%, the most common deletion site is 8p22, the positive rate of 38%. [Conclusion] M-FISH and CGH are powerful tools to study the genome of lung adenocarcinoma. The genes found in this experiment may represent the candidate genes related to the pathology and diagnosis of lung adenocarcinoma.