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为观察人ＦＧＦＲ－１基因变化与结肠癌发生的关系。采用ＲＴ－ＰＣＲ法从人肺成纤维细胞中钓取ＦＧＦＲ－１全编码区ｃＤＮＡ，用此ｃＤＮＡ探针与人肠癌及癌旁组织ＤＮＡ进行Ｓｏｕｔｈｅｒｎ印迹分析。结果表明：与癌旁组织相比结肠癌组织Ｓｏｕｔｈｅｒｎ印迹图谱发生了改变。提示ＰＧＦＲ－１基因变化与肠癌发生可能存在一定相关性。
To observe the relationship between human FGFR-1 gene expression and the development of colon cancer. The full-length cDNA of FGFR-1 was obtained from human lung fibroblasts by RT-PCR, and Southern blot analysis was performed using this cDNA probe with human colorectal cancer and paracancerous tissues. The results showed that the Southern blot of colon cancer tissue was altered compared to the paracancerous tissues. Tip PGFR-1 gene changes and the occurrence of colorectal cancer may be related to some.