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目的:利用免疫组织化学的方法探讨小G蛋白Ran在胃癌中的表达及临床意义。方法:利用免疫组织化学染色法研究74例胃癌组织标本(其中高分化25例,中分化24例,低分化25例)及其毗邻正常组织中Ran的表达情况,并分析该蛋白表达水平与临床病理参数之间的关联。结果:(1)Ran在胃癌组织中的染色强度明显高于正常组织。(2)在癌组织中Ran表达于胞核和胞浆,其中又以胞核为主,在正常组织中Ran主要表达于胞浆。(3)Ran的表达与患者年龄、性别无相关性(0.464、0.912),与肿瘤分化、TNM分期和转移与否有显著相关性(0.001、<0.001、<0.001)。结论:与正常组织相比,Ran在胃癌组织中的表达显著增高,并且与肿瘤分化和病理分期存在显著正相关,其可能作为胃癌新的分子标志物,在肿瘤的发生发展中发挥重要作用。
Objective: To investigate the expression of small G protein Ran in gastric cancer by immunohistochemistry and its clinical significance. Methods: Immunohistochemical staining was used to detect the expression of Ran in 74 cases of gastric cancer (25 cases were well differentiated, 24 cases were moderately differentiated, 25 cases poorly differentiated) and adjacent normal tissues. The expression of Ran in 74 cases of gastric cancer was analyzed. Correlation between pathological parameters. Results: (1) The staining intensity of Ran in gastric cancer tissues was significantly higher than that in normal tissues. (2) Ran was expressed in the nucleus and cytoplasm in the cancer tissues, which were mainly in the nucleus. Ran was mainly expressed in the cytoplasm in normal tissues. (3) There was no significant correlation between the expression of Ran and age and gender of patients (0.464, 0.912), and with tumor differentiation, TNM stage and metastasis (0.001, <0.001, <0.001). CONCLUSIONS: Compared with normal tissues, Ran expression in gastric cancer tissues is significantly increased, and it is positively correlated with tumor differentiation and pathological staging. As a new molecular marker of gastric cancer, Ran plays an important role in tumorigenesis and progression.