论文部分内容阅读
目的通过对ＨＩＶ１毒株ｇａｇ、ｔａｔ、ｅｎｖ基因的序列分析，阐明Ｄ亚型ＨＩＶ１毒株已在中国出现。方法从１名四川非洲回国劳务人员ＨＩＶ感染者（ＳＣ９７１２）淋巴细胞（ＰＢＭＣ）中提取前病毒ＤＮＡ，使用套式ＰＣＲ方法分别扩增ＨＩＶ１的ｇａｇ基因区、ｅｎｖ基因的Ｃ２Ｖ３区和ｔａｔ基因的第一外显子。结果发现ＳＣ９７１２在ｇａｇ区、ｅｎｖ区和ｔａｔ区与国际标准Ｄ亚型毒株的基因距离最近，其中在ｇａｇ区与Ｖ１２０３和Ｋ３１的基因离散率分别为９９４％和９５９％，在ｔａｔ基因区与ＵＧ２７４Ａ的基因离散率只有７５６％，在ｅｎｖ区与ＭＡＬ的基因离散率是１３８６％。系统树分析表明，ＳＣ９７１２与国际标准Ｄ亚型毒株、ＶＩ２０３、Ｋ３１、ＭＡＬ及ＵＧ２７４和Ｚ２７６等非洲流行株聚在一起。结论非洲Ｄ亚型毒株已随着援外劳务人员的回国传入中国。
Objective To analyze the sequence of HIV-1 strain gag, tat and env genes and elucidate that the D-type HIV-1 strain has appeared in China. Methods The provirus DNA was extracted from one SCMC HIV infected (SC9712) lymphocyte (PBMC) from a migrant worker in Sichuan Province, and the HIV-1 gag gene region, C2V3 region and tat gene of env gene were amplified by nested PCR The first exon. The results showed that SC9712 had the closest genetic distance to the international standard D subtype strains in the gag, env and tat regions, and the gene divergence rates in gag region and V1203 and K31 were 994% and 959%, respectively. The gene divergence rate of tat gene region and UG274A was only 756%, while that of env region and MAL gene was 1386%. Phylogenetic tree analysis showed that SC9712 clustered with the international standard D subtype strains, VI203, K31, MAL, UG274 and Z276 and other African epidemic strains. Conclusion The subtype D strains of Africa have been introduced into China with the return of aid workers.