Disinfection byproducts(DBPs)represent a ubiquitous source of chemical exposure in disin-fected water.While over 700 DBPs have been identified,the drivers of toxicity remain poorly understood.Additionally,ever evolving water treatment practices have led to a continually growing list of DBPs.Advancement of analytical technologies have enabled the identification of new classes of DBPs and the quantification of these chemically diverse sets of DBPs.Here we summarize advances in new workflows for DBP analysis,including sample preparation,chromatographic separation with mass spectrometry(MS)detection,and data processing.To aid in the selection of techniques for future studies,we discuss necessary considerations for each step in the strategy.This review focuses on how each step of a workflow can be opti-mized to capture diverse classes of DBPs within a single method.Additionally,we highlight new MS-based approaches that can be powerful for identifying novel DBPs of toxicologi-cal relevance.We discuss current challenges and provide perspectives on future research directions with respect to studying new DBPs of toxicological relevance.As analytical tech-nologies continue to advance,new strategies will be increasingly used to analyze complex DBPs produced in different treatment processes with the aim to identify potential drivers of toxicity.