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目的:研究精氨酸单糖苷(Arginyl-fructose,AF)对环磷酰胺(CTX)诱导的免疫抑制小鼠免疫功能的影响。方法:ICR小鼠100只,随机分成5组,即正常对照组(N),免疫抑制模型组(M),免疫抑制AF低、中、高剂量组(M-L、M-M、MH)。试验组连续给药28 d。免疫抑制组,每周前3天,腹腔注射80 mg/kg环磷酰胺,形成免疫抑制。末次给药12 h解剖后,测定免疫器官指数,AF对脾淋巴细胞转化及增殖、巨噬细胞吞噬功能、特异性Ig G抗体、血清中TNF-α、IL-2含量的影响。并通过实时荧光定量PCR检测TNF-α、IL-2基因表达的差异。结果:AF能够显著提高小鼠的胸腺指数和脾脏指数,促进脾淋巴细胞的自然转化与增殖,增强巨噬细胞吞噬功能,显著提高小鼠血清中特异性Ig G抗体含量,可显著提高小鼠血清中TNF-α、IL-2含量,实时荧光定量PCR检测结果显示,TNF-α、IL-2 mRNA能够良好表达。结论:AF能拮抗CTX免疫功能低下小鼠的免疫抑制作用。
Objective: To study the effect of arginyl-fructose (AF) on immune function in immunosuppressed mice induced by cyclophosphamide (CTX). Methods: One hundred ICR mice were randomly divided into five groups: normal control group (M), immunosuppressive model group (M) and immunosuppressive AF low, medium and high dose groups (M-L, M-M, MH). The experimental group was administered continuously for 28 days. Immunosuppression group, intraperitoneal injection of 80 mg / kg cyclophosphamide three days before the week, the formation of immunosuppression. After the last administration for 12 h, the immune organ index and the effect of AF on the splenic lymphocyte transformation and proliferation, macrophage phagocytosis, specific Ig G antibody, serum TNF-α and IL-2 levels were determined. Real-time fluorescent quantitative PCR was used to detect the difference of TNF-α and IL-2 gene expression. Results: AF could significantly increase the thymus index and spleen index of mice, promote the natural transformation and proliferation of splenic lymphocytes, enhance the phagocytosis of macrophages and significantly increase the specific Ig G antibody in serum of mice, The levels of TNF-α and IL-2 in serum and real-time fluorescence quantitative PCR showed that TNF-α and IL-2 mRNA were well expressed. Conclusion: AF can antagonize the immunosuppressive effect of CTX immunocompromised mice.