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研究ABCE1对肺癌(95-D和NCI-H446)细胞的作用.使用RNA干扰技术,抑制ABCE1基因的表达,通过Western blot分析及FACS检测,观察ABCE1基因对E-钙黏附蛋白在95-D/NCI-H446细胞表达的影响;运用transwell侵袭实验,观察M95-D/NCI-H446细胞侵袭力的变化.RNA干扰ABCE1基因后,实验组与对照组相比,在48h后可显著抑制肺癌(95-D和NCI-H446)细胞ABCE1蛋白的表达,同时,伴随E-钙黏附蛋白的高表达,以及细胞侵袭力的降低.ABCE1基因与E-钙黏附蛋白相关,抑制ABCE1基因可增加肺癌95-D/NCI-H446细胞的E-钙黏附蛋白的表达,减低细胞的侵袭力。
To investigate the effect of ABCE1 on the expression of E-cadherin in 95-D and NCI-H446 cells.METHODS: To investigate the effect of ABCE1 on the expression of E-cadherin in 95-D / NCI-H446 cells were detected by flow cytometry.The invasion ability of M95-D / NCI-H446 cells was observed by transwell invasion assay.Compared with the control group, the RNA interference of ABCE1 gene inhibited the invasion of lung cancer (95 -D and NCI-H446), accompanied by the high expression of E-cadherin and the decrease of cell invasiveness.ABCE1 gene is associated with E-cadherin, inhibiting ABCE1 gene may increase the expression of 95- Expression of E-cadherin in D / NCI-H446 cells, reducing cellular invasiveness.