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目的:通过动物实验,探讨氟引起学习记忆能力的改变及硒对氟的拮抗作用。方法:32只3周龄SD大鼠,雌雄各半,按体重随机分为4组。对照组每天给予去离子水灌胃,氟暴露组每天给予氟化钠20 mg/kg灌胃,加硒拮抗组氟化钠(20 mg/kg)灌胃同时自由饮用加入亚硒酸钠(2 mg/L)的去离子水,加硒组自由饮用去离子水加入亚硒酸钠(2 mg/L)。染毒3个月后Morris水迷宫测试学习记忆能力,处死前留取24 h尿液,测定血氟、尿氟含量以及大鼠脑组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。结果:①氟暴露组血氟含量较对照组明显升高(P<0.05),加硒拮抗组较氟暴露组明显下降,加硒组明显低于各组;与对照组比较,氟暴露组含量显著增高(P<0.05),加硒拮抗组尿氟明显高于氟暴露组。②氟暴露组脑组织SOD活性较对照组明显降低(P<0.05),加硒拮抗组较氟暴露组明显升高(P<0.05);氟暴露组脑组织MDA含量较对照组明显增加(P<0.05),加硒拮抗组较氟暴露组明显减少(P<0.05)。③水迷宫测试结果:氟暴露组逃逸潜伏期较对照组时间延长(P<0.05),加硒拮抗组较氟暴露组逃逸潜伏期明显缩短(P<0.05),加硒组较对照组逃逸潜伏期明显缩短(P<0.05)。结论:氟中毒可导致学习记忆能力下降,硒可拮抗氟引起的学习记忆能力下降。
OBJECTIVE: To investigate the changes of learning and memory ability induced by fluoride and the antagonistic effect of selenium on fluorine in animal experiments. Methods: Thirty-two 3-week-old SD rats were randomly divided into 4 groups according to body weight. The control group was given deionized water everyday. In the fluoride exposure group, sodium fluoride 20 mg / kg was orally administered daily, sodium selenite (20 mg / kg) mg / L) deionized water, plus selenium group drinking deionized water to join the sodium selenite (2 mg / L). Morris water maze was used to test the learning and memory abilities of rats after 3 months of exposure. The urine samples were taken for 24 hours before sacrifice. The content of blood fluoride and urinary fluoride and the activities of superoxide dismutase (SOD), malondialdehyde (MDA) )content. Results: (1) Compared with the fluoride exposed group, the blood fluoride level in fluoride exposure group was significantly higher than that in the control group (P <0.05), while the selenium exposure group was significantly lower than that in the fluoride exposure group. Compared with the control group, (P <0.05). Urinary fluoride in selenium-antagonistic group was significantly higher than that in fluoride-exposed group. (2) Compared with control group, the activity of SOD in the exposed group was significantly lower than that in the control group (P <0.05), and the content of MDA in the exposed group was significantly higher than that of the fluoride exposure group (P <0.05) <0.05), selenium antagonist group was significantly lower than the fluoride exposure group (P <0.05). ③ The results of water maze test showed that escape latency of fluoride exposure group was longer than control group (P <0.05), escape latency of fluoride-exposed group was significantly shorter than that of fluoride exposure group (P <0.05) (P <0.05). Conclusion: Fluorosis can lead to decreased ability of learning and memory, selenium can reduce the ability of learning and memory caused by fluoride.