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运用染色体显微分离技术构建了水稻第９号（Ｋ１０）染色体的ＤＮＡ文库，连接物转化大肠杆菌ＤＨ５α共获得４５０００多个重组克隆．随机选取了１００个重组克隆进行分析，插入序列的大小为８０～５００ｂｐ，平均２１０ｂｐ
The DNA library of rice chromosome 9 (K10) was constructed by chromosome microscopic separation technique. The conjugate was transformed into E. coli DH5α and more than 45,000 recombinant clones were obtained. Randomly selected 100 recombinant clones for analysis, the size of the inserted sequence is 80 ~ 500bp, an average of 210bp