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目的:探讨凋亡相关蛋白Survivin(生存素)和Caspase-3(半胱氨酸蛋白酶-3)在子痫前期患者与正常妊娠妇女胎盘组织中的表达规律及其在子痫前期发病过程中可能的机制。方法:随机选取子痫前期患者60例(轻度组25例,重度组35例)和正常妊娠孕妇30例作为病例组和对照组,应用免疫组织化学(SABC)法对3组胎盘的Survivin、Caspase-3进行标记,通过彩色病理图像分析系统对染色结果进行定量检测并分析。结果:①Survivin在子痫前期重度组表达低于轻度组及对照组,差异有统计学意义(P<0.01);轻度组与对照组比较,差异无统计学意义(P>0.05);②Caspase-3在子痫前期重度组表达高于轻度组及对照组,轻度组表达高于对照组,差异均有统计学意义(P<0.01);③Survivin与Caspase-3在3组胎盘组织中表达呈负相关(r=-0.370,P<0.05)。结论:子痫前期患者胎盘组织中凋亡相关蛋白Survivin表达下调、Caspase-3表达上调,Survivin及Caspase-3的表达变化可能与子痫前期的发病机制有关。
Objective: To investigate the expression of Survivin and Caspase-3 in placenta of preeclampsia and normal pregnant women and its possible role in the pathogenesis of preeclampsia Mechanisms. Methods: 60 cases of preeclampsia (25 cases in mild group and 35 cases in severe group) and 30 cases of normal pregnant women were selected randomly as the case group and the control group. The expression of Survivin, Caspase-3 was labeled, and the staining results were quantitatively detected and analyzed by color pathological image analysis system. Results: (1) The expression of Survivin in severe preeclampsia group was lower than that in mild group and control group (P <0.01); there was no significant difference between mild group and control group (P> 0.05); ② Caspase -3 in preeclampsia severe group was higher than in mild group and control group, mild group was higher than that in control group, the difference was statistically significant (P <0.01); ③Survivin and Caspase-3 in 3 groups of placenta Expression was negatively correlated (r = -0.370, P <0.05). CONCLUSIONS: The expression of Survivin and Caspase-3 in the placenta of preeclampsia patients is down-regulated while the expression of Survivin and Caspase-3 may be related to the pathogenesis of preeclampsia.