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目的:观察梅毒患者外周血单核细胞Toll样受体2表达与血清中IL-2、6含量变化及其意义。方法:采用流式细胞仪检测60例显性梅毒患者外周血单核细胞表面TLR2蛋白表达水平(荧光强度)以及TLR2阳性细胞百分比,ELISA法检测血清IL-2、6水平,并以60例健康者作为对照组。结果:①显性梅毒患者外周血单核细胞TLR2的表达水平较对照组显著升高且一期低于二期梅毒患者,差异均有统计学意义(P<0.05);TLR2+CD14+细胞的阳性率低于正常对照组且一期低于二期梅毒患者,差异均有统计学意义(P<0.05)。②显性梅毒患者IL-2、IL-6均高于对照组,一期患者IL-2高于二期但IL-6低于二期,所有差异有统计学意义(P<0.01)。③TLR2的表达水平与TLR2+CD14+阳性率、IL-2无明显相关性,与IL-6呈显著正相关;IL-2与IL-6呈显著负相关。④显性梅毒患者外周血单核细胞TLR2的表达水平、TLR2+CD14+阳性率、IL-2、IL-6男女之间的表达无差异。结论:梅毒感染时TLR2、IL-2、IL-6显著增高且与病程有关,但与性别无关。
OBJECTIVE: To observe the changes of Toll-like receptor 2 (PBMC) and IL-2β in serum of patients with syphilis and its significance. Methods: Flow cytometry was used to detect the expression of TLR2 protein (TLR2) and the percentage of TLR2 positive cells in peripheral blood mononuclear cells of 60 patients with dominant syphilis. The levels of serum IL-2 and 6 were detected by ELISA. As a control group. Results: (1) The expression of TLR2 in peripheral blood mononuclear cells of patients with dominant syphilis was significantly higher than that of the control group (P <0.05). The positive rate of TLR2 + CD14 + cells Rate lower than the normal control group and a lower than the second syphilis patients, the difference was statistically significant (P <0.05). ② The levels of IL-2 and IL-6 in patients with dominant syphilis were significantly higher than those in the control group (P <0.01). The expression of TLR2 was not significantly correlated with the positive rate of TLR2 + CD14 + and IL-2, but positively correlated with IL-6. There was a significant negative correlation between IL-2 and IL-6. The expression of TLR2, the positive rate of TLR2 + CD14 +, the expression of IL-2, IL-6 between male and female were not different between patients with dominant syphilis and peripheral blood mononuclear cells. Conclusion: The syphilis infection TLR2, IL-2, IL-6 was significantly higher and related to the course, but not sex.