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马来丝虫感染期幼虫(L_3)体外培养是了解丝虫幼虫在终宿主体内生长发育的重要手段之一,同时为收集代谢产物和蜕皮液以制备抗原和虫苗等进行免疫学研究以及药物筛选开辟新的研究领域。1960和1965年,Taylor、Sawyer等相继报道犬恶丝虫感染期幼虫体外培养可存活30天,并蜕皮进入四期幼虫;1979年Chen和Howeus报道彭亨丝虫L_3体外培养存活20天,1982年Wong等报道犬恶丝虫和彭亨丝虫L_3分别存活31～33天,且均可完成蜕皮。在我国尚未见有类似的报道。1983年5月我们实验室成功地建立了马来丝虫感染期幼虫
In vitro culture of larvae (L_3) of Malayi infection is one of the most important ways to understand the growth and development of filarial larvae in the final host. At the same time, immunological studies on the production of antigens and insects for metabolites and ecdysis were carried out, Screening open up new areas of research. In 1960 and 1965, Taylor and Sawyer et al. Reported that the larvae infected with D.oximax could survive for 30 days in vitro and molted into the fourth stage larvae respectively. In 1979, Chen and Howeus reported that the Paeonia lactiflora L_3 survived in vitro for 20 days in 1982 Reported such as Drosophila melanogaster and Paenibacterium filariasis L_3 survival of 31 to 33 days, and can complete the molting. No similar reports have been seen in our country. In May 1983, our laboratory successfully established larvae of Malayan filariasis