论文部分内容阅读
本文旨在研究广东人中ＧＳＴＭ１的遗传多态性与大肠癌的关系，同时选用能间接反映ＤＮＡ修复能力的Ｂｌｅｏｍｙｃｉｎ诱变剂敏感性试验，进行大肠癌的病例—对照研究。用ＰＣＲ方法检测ＧＳＴＭ１－的基因型，病例组为３６．８％（Ｎ＝１９）、对照组为２６．１％（Ｎ＝２３），差异无显著意义（Ｐ＞０．０５）；ｂｌｅｏｍｙｃｉｎ诱导的染色体平均断裂数（ｂｒｅａｋｓ／ｃｅｌ），病例组为０．７５±０．２９，对照组为０．４２±０．２４，差异有显著意义（Ｐ＜０．０５，且ｂ／ｃ＞０．８，在病例组的比例（６８％）高于对照组３倍，ＯＲ比为８．６７，ｂ／ｃ＞１．０，在病例组的比例（４７％）高于对照组４倍，ＯＲ比为６．６０。
This article aims to investigate the relationship between genetic polymorphisms of GSTM1 and colorectal cancer in Guangdong, and to use a Bleomycin mutagen sensitivity test that can indirectly reflect the DNA repair capacity, and conduct a case-control study of colorectal cancer. The genotypes of GSTM1- detected by PCR were 36.8% (N=19) in the case group and 26.1% (N=23) in the control group, with no significant difference (P>0.05); bleomycin induction The average number of broken chromosomes (breaks/cel) was 0.75±0.29 in the case group and 0.42±0.24 in the control group, with a significant difference (P<0.05, b/c>0. .8, in the case group (68%) was 3 times higher than the control group, the OR ratio was 8.67, b/c> 1.0, and the proportion in the case group (47%) was 4 times higher than in the control group. The OR ratio is 6.60.