论文部分内容阅读
目的体外研究人牙根尖乳头细胞(HAPCs)对人单核细胞系THP-1活化的影响及其分子机制。方法采用Transwell系统共培养HAPCs和THP-1,Real-time PCR检测THP-1中TNF-α、IL-12、IL-10及TGF-β1的mRNA表达量,流式细胞术检测共培养3 d THP-1中TNF-α的表达,通过Western blotting检测Stat3及Smad3磷酸化情况。结果 Real-time PCR结果显示,HAPCs抑制THP-1中TNF-α、IL-12的mRNA表达而促进IL-10、TGF-β1的mRNA表达(P<0.05);流式细胞术结果显示,共培养3 d的THP-1中TNF-α表达显著降低(P<0.05);Western blotting结果显示,共培养后HAPCs激活THP-1中的Stat3通路(P<0.05),而对Smad3通路无影响(P>0.05)。结论 HAPCs可能通过Stat3通路抑制THP-1活化。
Objective To investigate the effect of human apical apex papilla cells (HAPCs) on the activation of human monocytic cell line THP-1 in vitro and its molecular mechanism. Methods The expression of TNF-α, IL-12, IL-10 and TGF-β1 mRNA in THP-1 cells were detected by Real-time PCR and co-culture of HAPCs and THP-1 by Transwell system. Flow cytometry was used to detect the expression of THP- The expression of TNF-α in THP-1 was detected by Western blotting. Results Real-time PCR results showed that HAPCs inhibited the mRNA expression of TNF-α and IL-12 in THP-1 and promoted the mRNA expression of IL-10 and TGF-β1 (P <0.05). Flow cytometry The expression of TNF-α in THP-1 cultured for 3 d decreased significantly (P <0.05). Western blotting showed that HAPCs activated Stat3 pathway in THP-1 cells (P <0.05) and had no effect on Smad3 pathway P> 0.05). Conclusion HAPCs may inhibit THP-1 activation through Stat3 pathway.