应用杂交瘤技术，以２种抗原分别免疫ＢＡＬＢ／Ｃ小鼠。从３次细胞融合中，筛选出４株分泌高滴度、高特异性抗Ａ１血型物质单克隆抗体的杂交瘤细胞系。经多种免疫化学试验证明：其中Ｂ５２３－Ｈ１０、Ｄ２４３－Ｈ９２株杂交瘤扩大培养上清液仅凝集Ａ１红细胞，而不凝集Ａ２、Ｂ、Ｏ型红血胞。它们结合部位的结构互补于：人工接上脂肪链的Ａ－活性三糖。从而表明该２株单抗为首次获得的抗Ａ１亚型红细胞单克隆抗体。不仅为研究Ａ１亚型血本质提供了极好的材料，也为医学临床提供Ａ１亚型血分型试剂准备了条件。 BALB / C mice were immunized with two antigens using the hybridoma technique. From 3 cell fusion, 4 hybridoma cell lines secreting high titers and high specific monoclonal antibodies against the A1 blood group were screened. A variety of immunochemical tests proved that: B523-H10, D243-H92 hybridoma expanded culture supernatant only agglutinated A1 red blood cells, but not agglutination A2, B, O-type red blood cells. The structure of their binding site is complementary to: Artificially linked fatty-chain A-active trisaccharide. Which indicates that the two monoclonal antibodies are the first monoclonal antibodies against the A1 subtype erythrocyte. It not only provides an excellent material for studying the blood subtype of A1 subtype but also prepares clinical subtype A1 blood subtype reagent for medical clinic.