论文部分内容阅读
为分析黄曲霉毒素Ｂ１（ＡＦＢ１）、乙型肝炎病毒（ＨＢＶ）和丙型肝炎病毒（ＨＣＶ）在致人原发性肝细胞癌（ＨＣＣ）时可能的协同作用，对同份人ＨＣＣ组织分别采用高效液相色谱法、嵌套式ＰＣＲ及逆转录嵌套式ＰＣＲ检测ＡＦＢ１、ＨＢＶＤＮＡ和ＨＣＶＲＮＡ，检出率分别为６７．７％（２１／３１）、８８．２％（６０／６８）和１３．２％（９／６８）。其中，ＡＦＢ１和ＨＢＶＤＮＡ重叠检出率为５８．１％（１８／３１），ＨＢＶＤＮＡ和ＨＣＶＲＮＡ重叠检出率为８．８％（６／６８），由４例ＨＣＣ患者重叠检出ＡＦＢ１、ＨＢＶＤＮＡ和ＨＣＶＲＮＡ；同步检测对照组，３种危险因素全部阴性。结果表明，３种危险因素均与人类肝癌直接相关，并且存在着协同致癌效应
To analyze the possible synergistic effects of aflatoxin B1 (AFB1), hepatitis B virus (HBV), and hepatitis C virus (HCV) on human primary hepatocellular carcinoma (HCC), the cohorts of HCC were The detection rates of AFB1, HBVDNA, and HCV RNA using high performance liquid chromatography, nested PCR, and reverse transcription nested PCR were 67.7% (21/31), 88.2% (60/68), and 13.2% (9/68). Among them, the overlap detection rate of AFB1 and HBV DNA was 58.1% (18/31), the overlap detection rate of HBV DNA and HCV RNA was 8.8% (6/68), and AFB1, HBV DNA and overlap were detected in 4 patients with HCC. HCVRNA; synchronous detection of the control group, all three risk factors were negative. The results show that all three risk factors are directly related to human liver cancer and there is a synergistic carcinogenic effect.