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1996～ 1 998年从新疆各主要植棉区采集 3 0个野生型棉花黄萎病菌菌株 ,陕西省植保所提供 T-9、VD-8、安阳菌株及泾阳菌株 ,经在 WAC培养基上诱发培养 ,3 4个菌株共获得 4 3 1个突变体 ,其中 1 1 4个为Nit M,占 2 6.4 5%,3 1 7个为 Nitl,占 73 .55%。经营养体亲和性配对测试 ,3 2个菌株可分为 2个营养亲合群( VCGs) ,T-9与 VD-8属于亲合群 ( VCG1 ) ,其余 3 0个菌株属于亲合群 ( VCG2 ) ,即落叶型菌株与非落叶型菌株分属于不同的营养亲合群 ,还有 2个菌株即安阳菌株和 1 3 6团菌株因不与任何菌株亲和 ,故暂未定亲和群。由此可见 ,新疆棉花黄萎病菌的类型比较单一 ,除个别菌株外基本属于同一亲和群 ( VCG2 )
From 1996 to 1998, 30 wild-type strains of Verticillium dahliae were collected from Xinjiang’s main cotton-planting areas. T-9, VD-8, Anyang and Jingyang strains were obtained from Shaanxi Provincial Institute of Plant Protection, and were induced on WAC medium A total of 431 mutants were obtained from 34 strains, of which 11 4 were Nit M, accounting for 6.454%, and 317 were Nitl, accounting for 73.55%. Three commercial strains were divided into two groups (VCGs), VCGs (T-9 and VD-8), and VCG2 and the other 30 strains belonged to VCG2 , That is, the deciduous and non-deciduous strains belonged to different nutrient affinity groups, and two strains, Anyang and 136 strains, were not affinitive to any strains. Thus, the type of cotton Verticillium dahliae in Xinjiang is relatively single except for some isolates, belonging to the same affinity group (VCG2)