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目的观察慢性乙型肝炎病毒hepatitisBvirusHBV感染者树突状细胞dendriticcellsDC形态、表型和功能的改变。方法从13例慢性乙肝患者和11例健康人外周血中分离和培养DC观察DC的形态。用流式细胞仪检测DC的表面标志HLA-DR、CD1a、CD80和CD86的表达。用3H-TdR掺入法,检测DC诱导混合性淋巴细胞反应mixedleukocytesreactionMLR的能力。结果正常人的DC较慢乙肝患者的DC在形态上更为典型。前者的DC不规则,细胞可表达较多的HLA-DR、CD80和CD86分子P<0.05诱导MLR的能力也较强P<0.05。结论慢乙肝患者外周血DC处于不完全成熟状态,其免疫刺激能力较低。
Objective To observe the morphological, phenotypic and functional changes of dendriticcells DC in patients with chronic hepatitis B virus hepatitisBvirusHBV infection. Methods The morphology of DCs was isolated and cultured from peripheral blood of 13 patients with chronic hepatitis B and 11 healthy controls. The surface markers of DCs, HLA-DR, CD1a, CD80 and CD86, were detected by flow cytometry. The 3H-TdR incorporation assay was used to detect the ability of DC to induce mixed leukocytes reaction MLR. Results DC in chronic hepatitis B patients with chronic hepatitis B is more typical morphologically. The former DC is irregular, cells can express more HLA-DR, CD80 and CD86 molecules P <0.05 also has a strong ability to induce MLR P <0.05. Conclusion The peripheral blood DC in patients with chronic hepatitis B are not fully mature, and their immunostimulatory capacity is low.