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波纹杂毛虫(Cyclophragma undans Walker)幼虫感染波纹杂毛虫核多角体病毒(简称CuNPV)初期,行动呆滞,食欲不振,体色变为深褐色。死后内脏液化,体壁易破,流出茶色脓液,不臭。室内毒效测定,CuNPV0.4×10~8PIB/ml能使6—8龄波纹杂毛虫幼虫死亡率高达94.7％。 CuNPV的多角体呈三角形、四边形、五边形和不规则的多边形。大小为1.8—2.9μm,少数可达4.0μm。多粒包埋,多数为2—4个。病毒粒子杆状,大小为250—300×30—40nm。多角体蛋白质晶格为点线型,两点的中心距离约为4.4nm。晶格点的直径为2.5nm。病毒束外面有一层共同的疏松蛋白质膜包围,膜厚为15—20μm。病毒粒子经直接释放的DNA,在电镜下呈松弛的开环状分子和闭环状分子,平均长度为36.1μm。分子量为71.12×10~6道尔顿。经试验鉴定本病毒为CuNPV。 CuNPV的安全试验结果表明,对脊椎动物未发现不安全之处。
Cyclophragma undans Walker larvae initially infected with ribled caterpillars Polyhedrosis virus (CuNPV), sluggish, loss of appetite, body color turned dark brown. Visceral liquefaction after death, body wall easily broken, out of brown pus, not smelly. Indoor toxic effects determination, CuNPV 0.4 × 10 ~ 8PIB / ml 6-8 instar larvae larvae mortality up to 94.7%. Polyhedron of CuNPV is triangular, quadrilateral, pentagonal and irregular polygons. The size of 1.8-2.9μm, a few up to 4.0μm. Multi-grain embedding, the majority of 2-4. Viral particles rod-shaped, the size of 250-300 × 30-40nm. The polyhedrin protein lattice is a dotted line with a center distance of about 4.4 nm. The diameter of the lattice points is 2.5 nm. Virus layer outside the layer surrounded by a common loose protein membrane, film thickness 15-20μm. DNA directly released by virions, open-lobed molecules and closed-loop molecules under electron microscopy with an average length of 36.1 μm. The molecular weight of 71.12 × 10 ~ 6 Dalton. The experiment identified the virus as CuNPV. The safety test results of CuNPV show that no insecure problems have been found in vertebrates.