背景:发芽是成功定植和全身播散炭疽杆菌的关键一步。在体内芽胞的发芽很少有可利用的数据,目前还不清楚芽胞与宿主细胞的相互作用是否是芽胞开始发芽所必要的。方法:为了调查炭疽杆菌芽胞和皮肤组织之间的早期的相互作用,芽胞接种在豚鼠腹腔内的无细胞装置中或小鼠耳上。通过菌落形成单位计数和电子显微镜分析发芽及细菌的生长情况。结果:在豚鼠模型中,发芽发生在体内无细胞接 Background: Germination is a crucial step in the successful colonization and dissemination of B. anthracis. There is very little available data on the germination of sprouts in the body, and it is unclear whether the sporulation and host cell interactions are necessary for sprouting to begin to germinate. Methods: In order to investigate the early interactions between Bacillus anthracis spores and skin tissue, spores were seeded in acellular devices in the peritoneal cavity of guinea pigs or in the ears of mice. The germination and bacterial growth were analyzed by colony forming units and electron microscopy. Results: In the guinea pig model, germination occurred in vivo with no cell junction