论文部分内容阅读
作者应用滤纸片干血液分离恶性疟原虫DNA进行聚合酶链反应的方法对所罗门群岛的489份血样品进行检查.结果表明此方法具有1)用血量少,经自然干燥后,易于保存和运输.2)DNA分离的方法简便、快捷.3)PCR的检测极限为1.5个原虫/μl血.其次,其结果与姬姆萨染色方法的结果进行了比较.结果提示姬姆萨染色方法的结果与其检查者的技术水平密切相关.在大面积普查时容易出现漏诊及误诊、此外,也证明单片段基因扩增同样容易出现漏诊及误诊.“,”To observe the value of application of polymerase chain reaction for the diagnosis of malaria in addition to lands. The results show:1)Filter paper disks can be used converiently for the collection of blood to detect malaria In PCR.and negatives are higher in rate than that of another method.3)The sensitivity was 72~79% for the PCR-410bp and that was 62~79% for the 618bp and specificity was 71~78% and 83~87%,respectively.The sensitivity of PCR asimproved to 74~83% by a combination of Solomon Islands and Japan.4)Among 50 positive amples proved by these two examiners,the sensitivity for 41obp was 88% and that for618bp was 86%,the combination of both PCR was 92%.5)The detection limit for both PCR was nearly about 1.5 parasites/microlitre-blood(corresponding to 0.0000375%parasitemia level).