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美国副药典(NF)ⅩⅢ版对片剂中甲萘醌(Menadione)的测定方法常导致偏低和错误的结果。本文报导Johnson所推荐的亚硫酸氢钠甲萘醌制剂可采用分配层析法进行测定。此法系以样品溶液为固定相,杂质被分离洗脱后,以氨-氯仿(1∶25)溶液使亚硫酸盐分解洗出,然后将游离的甲萘醌用分光光度法测定。这个方法也可以很简便地分析甲萘醌制剂,为先使甲萘醌和亚硫酸氢钠作用生成亚硫酸氢钠甲萘醌后,再用分配层析法处理测定之。
The determination of Menadione in tablets by the U.S. Pharmacopoeia (NF), XIII, often leads to low and erroneous results. This article reports that Johnson’s recommended sodium bisulfite menadione formulations can be assayed by partition chromatography. In this method, the sample solution is used as the stationary phase. After the impurities are separated and eluted, the sulfite is eluted and eluted with an ammonia-chloroform (1:25) solution, and the free menadione is measured spectrophotometrically. This method can also be very simple analysis of menadione preparations, for the first menadione and sodium bisulfite to generate sodium bisulfite menadione, and then measured by the distribution chromatography.