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目的:研究紫杉醇脂质体对B细胞增殖及免疫功能的抑制作用。方法:分别用不同浓度紫杉醇脂质体(1,2.5,5,10,20ng·mL_1)对体外培养的多发性骨髓瘤细胞XG-7作用24h后,采用CCK-8活细胞计数法检测细胞增殖,同时用ELISA法检测细胞培养上清IgG水平及BAFF含量。结果:紫杉醇脂质体作用24h后,对XG-7细胞增殖具有抑制作用,细胞增殖活性随浓度增加逐渐降低,紫杉醇脂质体浓度为5ng·mL~(-1)以上时对细胞增殖有明显的抑制作用,且细胞培养上清的IgG含量明显减少,与空白对照组相比差异有显著性(P<0.05),BAFF含量随药物浓度增加而有所降低,但降低不明显,与对照组相比差异无统计学意义(P>0.05)。结论:紫杉醇脂质体可以抑制B细胞增殖并且抑制其IgG分泌,因此可能具有治疗自身免疫性疾病潜在的临床价值。
Objective: To study the inhibitory effect of paclitaxel liposomes on the proliferation and immune function of B cells. Methods: After treated with different concentrations of paclitaxel liposomes (1, 2.5, 5, 10 and 20 ng · mL -1) for 24 h, respectively, the cells were cultured in vitro for 24 hours. Cell viability was detected by CCK-8 viability cell counting At the same time, the cell culture supernatant IgG level and BAFF content were detected by ELISA. Results: After treated with paclitaxel liposome for 24 h, the proliferation of XG-7 cells was inhibited. The proliferation activity of paclitaxel liposome decreased gradually with the increase of the concentration of paclitaxel. The paclitaxel liposomes had obvious proliferation when the liposome concentration was above 5 ng · mL -1 (P <0.05). The content of BAFF decreased with the increase of drug concentration, but the decrease was not obvious. Compared with the control group Compared with no significant difference (P> 0.05). Conclusion: Paclitaxel liposomes can inhibit the proliferation of B cells and inhibit the secretion of IgG, so it may have potential clinical value in the treatment of autoimmune diseases.