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体内评价新潜在因子的抗丝虫作用耗时而且不经济。发展快速、可靠和低成本的体外筛选系统极有必要。本文作者报道一种以虫体的能动性和四唑盐(MTT)比色试验作为参数来检测杀丝虫药作用的体外试验系统。 对六月龄的雄性Mastomys coucha通过皮下接种感染性棘丝虫幼虫90±10天后从其皮下组织分离出棘丝虫成虫,用过滤器分离M.coucha的心脏和肺部微丝蚴。成虫和
The anti-filariasis effect of evaluating new potential agents in vivo is time consuming and uneconomical. It is imperative to develop a rapid, reliable and cost-effective in vitro screening system. The authors report an in vitro assay system for the detection of the killing effect of mycotoxins on the basis of the motility of the parasites and the MTT colorimetric assay. Six-month-old male Mastomys coucha were separated from their subcutaneous tissue by subcutaneously inoculated infectious Chrysene larvae for 90 ± 10 days, and filters were used to separate M.couch’s heart and lung microfilariae. Adult and