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自从分散肝组织培养肝细胞的方法于16年前建立以来,许多学者对原代肝细胞的体外培养,尤其是对如何建立适合肝细胞体外生长的条件进行了深入研究.但迄今为止,如何使原代培养中的肝细胞不失去原有的功能,并保留对各种激素的反应,仍是一个悬而未决的问题.肝脏的几类细胞中,起主要功能作用的是肝实质细胞,占肝脏细胞总数的70%.原代培养的第一步就是将这些实质细胞分离出来.Berry等于1969年首创的胶原酶灌洗肝脏的方法使这项工作获得成功.用该方法获取的肝细胞几乎为纯的实质细胞,不仅数量多,而且保留了肝细胞的各种功能.70年代初期,Bissell报道了体外研究肝功能和激素反应的所谓“最
Since the establishment of a method to disperse hepatocytes in hepatocytes has been established 16 years ago, many scholars have conducted in-depth studies on how to culture primary hepatocytes in vitro, and in particular, how to establish conditions suitable for the growth of hepatocytes in vitro. Primary cultured liver cells do not lose their original function, and retain the response to various hormones, is still an open question.In the liver of several types of cells, play a major role in the function of liver parenchymal cells, accounting for liver cells 70% of the total number of primary culture is the first step is to separate these parenchymal cells.Berry equivalent to the first in 1969 collagenase lavage of the liver method to make this work was successful using this method to obtain almost pure liver cells Of the parenchymal cells, not only in large numbers, but also retain the various functions of the hepatocytes. In the early 1970s, Bissell reported the so-called ”best" study of liver function and hormone responses in vitro