选用经高温灭活的香菇原养型双核原生质体作供体亲本 ,以带有脯氨酸( Prol)营养缺陷型标记的凤尾菇单核原生质体为受体亲本 ,对其原生质体融合情况进行研究。结果发现 ,两亲本原生质体的融合再生率为 0 .1 0 6%,部分融合子菌丝细胞多核 ,无锁状联合。对该融合子再进行原生质体分离、再生 ,得到了具有新的生理特性的菌株材料。表明这种单双核原生质体融合是实现食用菌属间远缘杂交育种的有益探索 The protoplasts from the prototrophic protoplasts of mushrooms inactivated by high temperature were selected as donor parents and protoplast protoplasts of Przewalsia mushrooms bearing Prol auxotrophic markers were used as the recipient parents. research. The results showed that the amphiploid protoplasts fusion regeneration rate was 0.166%, some fusion mycelial multinuclear cells, no lock-like combination. Protoplasts were separated and regenerated from the fusion, and the strain material with new physiological characteristics was obtained. It is indicated that the fusion of protoplasts with single dinuclear protoplasts is a useful exploration to realize distant hybridization between edible fungi