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0 引言 我们建立了巢式竞争 PCR定量方法进行血清HBV DNA定量扩增 [1 ] .在该实验中我们采用了简便的长引物引导扩增方法制备了特殊的巢式竞争 DNA模板 .1 材料和方法1. 1 材料 我们对 HBV DNA C区 186 5 - 2 45 8区段进行巢式扩增定量 ,而巢式扩增片段长度为 5 5 9bp.按
0 Introduction We have established a nested competitive PCR quantification method for the quantitative amplification of serum HBV DNA.1 In this experiment we used a convenient long primer-directed amplification method to prepare a special nested competitive DNA template.1 Materials and Method 1.1 Materials We performed nested amplification of the 186 5-2 458 segment of the HBV DNA C region, while the length of the nested amplified fragment was 559 bp.