根据菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)不同分离株外壳蛋白基因(coat protein,CP)的保守序列,设计了特异性引物与TaqMan MGB荧光探针,建立了BPMV的实时荧光RT-PCR检测方法。该方法与酶联免疫检测方法相比,灵敏度提高了100倍,具有快速、灵敏和高特异性的优点,适于对BPMV的快速检测。应用该方法,对来自美国不同地区的携带有BPMV的大豆样品进行检测,均能够得到BPMV的典型扩增曲线,表明引物与探针对BPMV不同分离株具有良好的简并性。 According to the conserved sequence of coat protein (CP) gene of Bean pod mottle virus (BPMV), specific primers and TaqMan MGB fluorescent probe were designed and the real-time fluorescent RT-PCR Detection method. Compared with enzyme-linked immunosorbent assay (ELISA), this method has the advantage of 100-fold higher sensitivity, rapidity, sensitivity and specificity. It is suitable for the rapid detection of BPMV. Using this method, we can get typical BPMV amplification curves of soybean samples carrying BPMV from different regions of the United States, indicating that primers and probes have good degeneracy for different BPMV isolates.