论文部分内容阅读
应用脑立体定位技术微量注射6-OHDA于兔右侧纹状体内。术后每周观察以阿扑吗啡(Apomorphin,APO)诱导的旋转行为,并于术后6周处死兔,以黑质酪氨酸羟化酶(Tyrosine hydroxylase,TH)免疫组化染色,观察黑质多巴胺能神经元的形态、结构及数量变化。结果表明,部分兔在术后即出现行动迟缓、躬身、易激怒等异常行为。术后6周时,20只兔中有16只在阿扑吗啡诱导后30min内的平均旋转圈数大于7r/min,达到成功模型标准。模型成功率达到80%。TH免疫组化染色可见正常对照组、假手术组及模型组未损侧黑质内有胞浆浓染、突起明显的TH免疫反应阳性神经元分布,神经元数量较多,轴突长度较长,且3者差异不显著(P>0.05);而模型组损毁侧黑质内TH免疫反应阳性神经元与上述3者相比,数目明显减少(P<0.05),残存的细胞染色较浅,胞体轮廓和突起均不清晰,轴突长度明显变短(P<0.05)。结果提示,将6-OHDA注射于兔单侧纹状体是一种制备帕金森病(Parkinson’s disease,PD)模型的有效方法,此法操作简便,动物死亡率低,模型制作成功率高。
Microinjection of 6-OHDA into the right striatum of rabbit using brain stereotactic technique. The rabbits were sacrificed at 6 weeks after operation, and the rotational behavior induced by apomorphin (APO) was observed every week. The rabbits were sacrificed 6 weeks after the surgery and immunohistochemical staining was performed with tyrosine hydroxylase (TH) Morphological, structural and quantitative changes of dopaminergic neurons. The results showed that some rabbits appeared in the postoperative slow, bending, irritability and other abnormal behavior. At 6 weeks after operation, 16 out of 20 rabbits achieved an average of more than 7 r / min of rotation within 30 min after apomorphine induction, reaching the standard of success model. Model success rate reached 80%. TH immunohistochemical staining showed normal control group, sham operation group and untreated group of non-lesion side of the substantia nigra in cytoplasm, marked prominent TH immunoreactive positive neurons distribution, the number of neurons, axon length longer (P> 0.05). Compared with the above three groups, the number of TH immunoreactive positive neurons in the lesioned substantia nigra in the model group was significantly decreased (P <0.05), and the remaining cells were stained lightly, The outline of the cell body and the protrusion were not clear, and the axon length was significantly shorter (P <0.05). The results suggest that injection of 6-OHDA into rabbit unilateral striatum is an effective method for the preparation of Parkinson’s disease (PD) model. The method is simple, animal mortality is low, and the model making success rate is high.