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[目的 ]探讨福建输入性疟疾的分子流行病学特征 ,追踪传染源。[方法 ]病人血样用套式PCR RFLP检测 ,扩增PvMSP 1基因ICB5 ICB6片段 ,测定DNA序列并进行BLAST相似性搜索和进化树分析。 [结果 ] 16例镜检确诊的间日疟病人血样经套式PCR RFLP检测 ,7份属Sal 1型 ,1份为Belem型 ,8份为重组Ⅲ型。对其中 7份样本PCR产物进行DNA直接测序 ,发现没有两个DNA序列完全相同 ,PvMSP 1基因ICB5 ICB6片段存在高度多态性 ,DNA序列搜索结合流行病学调查可初步确定感染来源。 [结论 ]PvMSP 1基因用于疟疾基因分型和判断感染来源有一定价值 ,可作为疟疾分子流行病学调查的方法之一。
[Objective] To investigate the molecular epidemiological characteristics of imported malaria in Fujian and follow up the source of infection. [Methods] The blood samples of patients were detected by nested PCR RFLP, and the fragment of ICB5 ICB6 of PvMSP 1 gene was amplified. The DNA sequence was determined and BLAST similarity search and phylogenetic tree analysis were performed. [Results] The blood samples of 16 cases of malaria patients diagnosed by microscopic examination were detected by nested PCR RFLP, 7 were Sal type 1, 1 was Belem type and 8 were recombinant type III. Seven of the PCR products were directly sequenced by DNA. No two DNA sequences were identical, and there was a high degree of polymorphism in the ICB5 ICB6 fragment of PvMSP1 gene. DNA sequencing and epidemiological investigation could initially identify the source of infection. [Conclusion] The PvMSP 1 gene is of some value in genotyping malaria and judging the source of infection, and may be used as one of the methods for molecular epidemiological investigation of malaria.