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自杂交稻推广种植以来,种子部门普遍通过南繁来鉴定杂交稻种子纯度.这种方法在人力、物力和时间上都不经济,因此,寻求一个更经济有效的鉴定办法,是当前发展杂交稻生产的迫切需要.本文介绍利用酯酶同工酶分析法鉴定杂交稻种子纯度的技术及其可行性.实验依据同工酶技术在水稻杂交优势与品种分类上利用已有不少文献报道.龚蓁蓁等运用酯酶同工酶分析高粱稻及其亲本,发现杂交子代中有与亲本异同的酶带.陆士伟等以酯酶同工酶E_3~S和E_3~F谱带作为杂交稻早期纯度测定的手段.易琼华等研究了酯酶同工酶酶带
Since the popularization of hybrid rice cultivation, the seed department has generally identified the purity of hybrid rice seeds through the South Fan. This method is not manpower, material and time economy, therefore, to find a more cost-effective identification method is the current development of hybrid rice The urgent need of production.This article describes the use of esterase isozyme analysis of hybrid rice seed purity technology and its feasibility.Experiment based on isozyme technology in the hybridization advantages and varieties of rice utilization have been reported in many literature. Such as the use of esterase isozyme analysis of sorghum rice and its parents and found that hybrid progenies have the same with the parents of the enzyme band.Lu Shiwei et al esterase isoenzyme E_3 ~ S and E_3 ~ F band as early hybrid rice purity determination Yi Qionghua et al. Studied esterase isozyme bands