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目的:用毛细管区带电泳-间接紫外检测技术检测氨基糖苷类抗生素中硫酸根。方法:采用非涂层弹性石英毛细管;背景电解质为含0.2 mmol.L-1十六烷基三甲基溴化铵的15 mmol.L-1铬酸水溶液(用三羟甲基氨基甲烷调节pH至8.1);操作电压:-20 kV;检测波长:276 nm(间接检测);电泳过程中在进样端始终外加适当的压力使基线稳定。结果:本文方法的线性范围为45～150μg.mL-1;定量限约为6μg.mL-1;硫酸根与内标峰面积比的RSD为0.6%(n=10);测定结果与采用英国药典方法的测定结果一致。结论:本方法适用于对氨基糖苷类抗生素中的硫酸根的测定。
Objective: To detect sulfate in aminoglycoside antibiotics by capillary zone electrophoresis with indirect UV detection. METHODS: Uncoated flexible silica capillary tubes were used. The background electrolyte consisted of 15 mmol·L-1 aqueous chromic acid solution containing 0.2 mmol·L -1 cetyltrimethylammonium bromide (adjusted with trimethylamidomethane To 8.1). Operating voltage: -20 kV. Detection wavelength: 276 nm (indirect detection). During electrophoresis, a suitable pressure is always applied at the injection end to stabilize the baseline. Results: The linear range of this method was 45 ~ 150μg.mL-1, the limit of quantification was 6μg.mL-1, and the RSD of sulfate and internal standard peak area was 0.6% (n = 10) Pharmacopoeia method of determination of the same. Conclusion: This method is suitable for the determination of sulfate in aminoglycoside antibiotics.