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最早诊断HCV感染的方法是检测抗c 100-3抗原的抗体,其敏感性较低。现编码HCV核壳互补DNA(cDNA)的22kD抗原(c22)已被抽提成功,且使用重组杆状病毒以表达c 22的ELISA法已确立,据报告其对HCV感染的诊断比抗c 100-3检测更敏感、更特异。因此作者在慢性肝病患者和献血员中,对这两种HCV检测方法作了对比研究。病人和方法:1989.1～1990.10期间,随访各种慢性肝病患者共184例。慢性NANB肝炎组97例,其中静脉药瘾者22例,有输血或血制品史者17例,无易患因素者58例。肝活检均符合NANB肝炎诊断标准。其他肝病组包括慢性HBV感染26例,自身免疫性肝
The earliest method of diagnosing HCV infection was to detect anti-c 100-3 antigen antibodies, which were less sensitive. The 22kD antigen (c22) now encoding the HCV core-shell complementary DNA (cDNA) has been successfully extracted and an ELISA using recombinant baculovirus to express c22 has been established and its diagnosis of HCV infection is reported to be more resistant than c100 -3 Detection is more sensitive and more specific. The authors therefore compared these two HCV assays in patients with chronic liver disease and blood donors. Patients and Methods: A total of 184 patients with various chronic liver diseases were followed up from 1989.1 to 1990.10. 97 cases of chronic NANB hepatitis group, of which 22 cases of intravenous drug addiction, history of transfusion or blood products in 17 cases, 58 cases without predisposing factors. Liver biopsy are in line with NANB hepatitis diagnostic criteria. Other liver disease group, including chronic HBV infection in 26 cases, autoimmune liver