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目的了解龙岩市健康儿童肠道病毒(Enterovirus,EV)携带状况,加强EV的监测及其相应疾病的预防。方法对龙岩市疾控中心2013~2014年收集的健康儿童粪便标本经RD细胞分离培养病变后应用荧光定量PCR方法进行毒株的鉴定。结果检测466份标本,经RD细胞分离,对培养物进行荧光定量PCR检测,健康儿童肠道病毒阳性22份,阳性率为4.72%;市区、农村健康儿童肠道病毒阳性率分别为1.43%、7.42%(P<0.01);学龄前儿童与学龄儿童肠道病毒阳性率分别为8.63%、1.86%(P<0.01)。22份肠道病毒通用引物核酸阳性标本中4份EV 71阳性,5份CVA 16同时阳性,3份EV 71与CVA 16同时阳性,3份CVB 5阳性,2份CVA10阳性;5份未定型。结论龙岩市健康儿童中学龄前儿童肠道病毒阳性率高于学龄儿童。
Objective To understand the carrying status of Enterovirus (EV) in healthy children in Longyan City, and to strengthen the monitoring of EV and the prevention of its corresponding diseases. Methods Stool samples from healthy children collected from 2013 ~ 2014 in Longyan CDC were isolated and cultured with RD cells. The strains were identified by fluorescence quantitative PCR. Results 466 samples were detected by RD cells, and the culture was detected by real-time PCR. The positive rate of enterovirus in healthy children was 22.2%, the positive rate was 4.72%. The positive rates of enterovirus in urban and rural children were 1.43% , 7.42% (P <0.01). The positive rates of enterovirus in preschool children and school-age children were 8.63% and 1.86% respectively (P <0.01). Four EV71 positive samples and four CVA 16 positive samples from positive samples of enterovirus universal primers were positive at the same time. Three copies of EV 71 and CVA 16 were positive at the same time. Three samples were positive for CVB 5 and two samples were positive for CVA10. Conclusion The positive rate of enterovirus in preschool children in healthy children in Longyan is higher than that in school-age children.