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目的 在单细胞水平上，研究类风湿性关节炎（ＲＡ）患者滑液及外周血中Ｔ淋巴细胞上ＣＣＲ５及ＣＸＣＲ３的表达，并结合临床资料分析其在ＲＡ发病中的可能作用机制及临床应用。方法 分离１５例ＲＡ患者的滑液单个核细胞（ＳＦＭＣ）、外周血单个核细胞（ＰＢＭＣ），及１５正常人ＰＢＭＣ（对照），以三色荧光素标记物进行流式细胞术分析Ｔ细胞上ＣＣＲ５及ＣＸＣＲ３的表达。结果 与ＰＢＭＣ相比较，ＲＡ患者ＳＦＭＣ中Ｔ细胞上ＣＣＲ５及ＣＸＣＲ３的表达显著增高（特别是ＣＣＲ５）；而ＣＸＣＲ３的表达个体差异较大；与正常人相比较，ＲＡ患者初发或活动期未治时，ＰＢＭＣ中ＣＣＲ５及ＣＸＣＲ３的表达明显增多。 ＣＣＲ５及ＣＸＣＲ３的表达率与患者的ＥＳＲ及ＣＲＰ相关。结论 ＣＣＲ５＋Ｔ细胞积聚在ＲＡ患者的病变关节内，且与ＲＡ的病情密切相关。
Objective To study the expression of CCR5 and CXCR3 on synovial fluid and peripheral blood T lymphocytes in patients with rheumatoid arthritis (RA) at the single cell level and to analyze its possible mechanism and its clinical application in RA with clinical data . Methods The synovial fluid mononuclear cells (SFMCs), peripheral blood mononuclear cells (PBMCs) and 15 normal PBMCs from 15 patients with RA were isolated and analyzed by flow cytometry using Tricolor fluorescein-labeled T cells CCR5 and CXCR3 expression. Results Compared with PBMC, the expression of CCR5 and CXCR3 on T cells in SFMCs was significantly increased in RA patients (especially CCR5), while the expression of CXCR3 was significantly different between the two groups. Compared with normal controls, the incidence of initial or active RA patients , PBMC CCR5 and CXCR3 expression was significantly increased. The expression rates of CCR5 and CXCR3 correlated with ESR and CRP in patients. Conclusion CCR5 + T cells accumulate in the diseased joints of patients with RA, and are closely related to the condition of RA.