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采用制霉菌素穿孔膜片箝技术，研究了锌离子（Zn2+）对急性分离的大鼠骶髓后连合核神经元GABAA受体介导电流的作用。结果表明：（1）在箝制电压为-40mV时，GABA可通过GABAA受体介导产生内向电流；（2）此电流可被Zn2+呈非电压依赖性可逆地阻断；（3）在Zn2+存在的情况下，GABA的浓度-效应曲线平行右移。上述结果提示，Zn2+可能通过变构调控机制对GANAA介导的反应产生抑制作用。
The effects of zinc ion (Zn2 +) on the GABAA receptor-mediated currents in acutely isolated rat sacral spinal cord commissural nucleus neurons were studied using nystatin-piercing patch-clamp technique. The results showed that: (1) GABA can generate an inward current through GABAA receptor at a clamping voltage of -40 mV; (2) this current can be reversibly blocked by Zn2 + in a voltage-independent manner; (3) The GABA concentration-response curves shift right parallel. The above results suggest that Zn2 + may inhibit the GANAA-mediated response through the allosteric regulation mechanism.