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【目的】探讨单侧纹状体内四点注射6-羟基多巴胺(6-Hydroxydopamine,6-OHDA)建立大鼠帕金森(Parkinson’s disease,PD)模型的可行性与成功率。【方法】48只SD大鼠随机分为对照组(n=12)和PD模型组(n=36)。PD模型组通过立体定位技术于纹状体内四点注射6-OHDA诱导PD疾病,于2周后先后进行阿扑吗啡诱导旋转行为学测试和11C-CFT Micro PET/CT显像以及免疫荧光染色法计数大鼠黑质致密部酪氨酸羟化酶(tyrosine hydroxylase,TH)阳性的多巴胺能神经元总数的变化,以评定PD模型的成功率。【结果】~(11)C-CFT Micro PET/CT显像结果显示,与对照组相比,PD模型组的11C-CFT损伤侧/健侧的放射性摄取比值(0.581±0.061,P<0.05)以及损伤侧/健侧的TH阳性多巴胺能神经元数量比值(0.514±0.085,P<0.05)均显著下降。阿扑吗啡诱导行为学旋转评定大鼠PD模型成功率为86.1%。【结论】纹状体内四点注射6-OHDA建立PD模型的方法可有效模拟人类PD多巴胺能神经元的退变减少,且成功率高(86.1%)。
【Objective】 To investigate the feasibility and success rate of establishing a rat model of Parkinson’s disease (PD) by injecting 6-Hydroxydopamine (6-OHDA) into the unilateral striatum. 【Methods】 Forty-eight SD rats were randomly divided into control group (n = 12) and PD model group (n = 36). PD model group was induced PD disease by four-point injection of 6-OHDA in the striatum by stereotaxic technique. Apomorphine-induced rotational behavior test and 11C-CFT Micro PET / CT imaging and immunofluorescence staining were performed two weeks later The total number of tyrosine hydroxylase (TH) -positive dopaminergic neurons in the substantia nigra pars compacta was counted to assess the success rate of the PD model. 【Results】 The results of ~ (11) C-CFT Micro PET / CT imaging showed that the 11C-CFT lesion side / contralateral radioactivity uptake ratio (0.581 ± 0.061, P <0.05) (0.514 ± 0.085, P <0.05) of TH-positive dopaminergic neurons in lesion side and contralateral side. Apomorphine induced behavioral rotation of rat model of PD success rate of 86.1%. 【Conclusion】 The method of 4-point injection of 6-OHDA in striatum to establish PD model can effectively reduce the degeneration of human PD dopaminergic neurons with a high success rate (86.1%).