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目的探索恶性疟原虫对复方新抗疟药双氢青蒿素/哌喹的敏感性现场体外监测方法 ,以指导药物敏感性监测。方法参照WHO推荐的Rieckmann体外微量测定法,在自制双氢青蒿素/哌喹涂药板中测定2种药物单方以及复方的敏感性。结果单一药物板测定成功率分别为76.92%和75.00%,复方涂药板测定成功率为76.92%;两药联用疟原虫抑制率明显高于单方,单方和复方各井疟原虫抑制率均随药物浓度的增高而依次上升。两药联用可明显减少药物用量,且两药协同降低哌喹使用比例。结论自制药物测定板稳定、灵敏、重复性好、简便易行,可现场推广使用;单方青蒿素类药物以及哌喹耐药性有上升趋势,两药联用时明显减少药物用量,可能延缓抗药性的产生;双氢青蒿素与哌喹两药组方最佳摩尔浓度比例为1∶3.5～4.4。
Objective To explore an in vitro monitoring method for susceptibility of Plasmodium falciparum to compound new antimalarial drug dihydroartemisinin / piperaquine to guide drug sensitivity monitoring. Methods According to the WHO recommended Rieckmann in vitro microdilution assay, the sensitivity of two drugs in one side and in combination was determined in homemade dihydroartemisinin / piperaquine coated tablets. Results The success rate of single drug plate determination was 76.92% and 75.00%, respectively, and the success rate of compound coated plate was 76.92%. The inhibition rates of two drugs combined with Plasmodium were significantly higher than those of unilateral, single and compound wells Drug concentration increased in turn increased. Combination of two drugs can significantly reduce the amount of drugs, and two drugs synergistic to reduce the use of piperaquine proportion. Conclusion The self-made drug assay plate is stable, sensitive and reproducible. It is simple and easy to use and can be widely used in the field. The unilateral artemisinin and piperine drug resistance are on the rise. The combined use of the two drugs significantly reduces the dosage of the drug and may delay the anti- Drug-induced; dihydroartemisinin and piperaquine two groups the best molar ratio of 1: 3.5 ~ 4.4.