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目的:研究小柴胡汤对四氯化碳/乙醇诱发小鼠肝癌肠-肝-脑损伤的影响。方法:肝癌模型组:腹部皮下注射25%四氯化碳-橄榄油溶液(5 mL·kg-1,2次/周)和自由饮用8%乙醇溶液4周,自由饮用四氯化碳(0.5%)-乙醇(8%)溶液20周;肝癌肝郁脾虚模型组:在肝癌模型基础上,夹尾(30 min.d-1)、孤养及隔日禁食4周,自由饮用四氯化碳(0.5%)-乙醇(8%)溶液、孤养和隔日禁食20周;小柴胡汤8 g·kg-1·d-1组:肝癌肝郁脾虚模型制作4周后灌小柴胡汤8周。监测死亡率、体重和症状,通过积分系统估计肠、肝、脑病理损伤程度。结果:肝癌肝郁脾虚组死亡率显著高于肝癌组(分别为70%和35%),小柴胡汤治疗后显著降低;肝癌组死亡小鼠存在明显的小肠、盲肠、肝脏损害、盲肠积粪及脑质量增加,肝癌肝郁脾虚组死亡小鼠以上表现更明显,小柴胡汤治疗后显著改善;肝癌肝郁脾虚组肝癌发生率显著高于肝癌组(分别为67%和46%),小柴胡汤治疗后显著降低;肝癌组存活小鼠存在明显的小肠、盲肠、肝脏损伤(增生为主),脑质量略降低,肝癌肝郁脾虚组存活小鼠以上表现更明显,小柴胡汤治疗后显著改善。相关分析发现,死亡小鼠和存活小鼠小肠损害、盲肠损害、盲肠积粪、肝损害、脑质量之间均呈显著正/负相关。结论:小柴胡汤通过降低肠-肝-脑损伤及改善肠-肝-脑互作用,抵御肝郁脾虚和四氯化碳/乙醇诱导的肠-肝-脑损伤和炎症,降低四氯化碳/乙醇诱发小鼠肝癌时的死亡率和肝癌发生率,其分子机制有待进一步阐明。
Objective: To study the effect of Xiao Chai Hu Tang on carbon tetrachloride / ethanol-induced hepatoma intestinal-liver-brain injury in mice. Methods: The models of liver cancer were divided into two groups: the abdomen was injected subcutaneously with 25% carbon tetrachloride - olive oil solution (5 mL · kg-1, 2 times / week) %) -ethanol (8%) solution for 20 weeks. Liver cancer and spleen deficiency model group: On the basis of liver cancer model, the rats were fasted for 30 min.d-1, fasted and fasted for 4 weeks every other day. (0.5%) - ethanol (8%) solution, isolated and fasted for 20 weeks every other day; Xiao Chai Hu Tang 8 g · kg-1 · d-1 group: 8 weeks. Mortality, weight, and symptoms were monitored, and the degree of pathological damage in the intestine, liver and brain was estimated by the integral system. Results: The mortality of hepatocellular carcinoma with liver depression and spleen deficiency group was significantly higher than that of liver cancer group (70% and 35%, respectively), and Xiaochaihu decoction decreased significantly after treatment. Liver, tumor and cecum, (P <0.05), and the brain mass increased, the liver cancer and liver spleen deficiency group more obvious death of mice, Xiao Chai Hu Tang significantly improved after treatment; liver cancer liver spleen deficiency group was significantly higher than the incidence of liver cancer group (67% and 46% Hu Tang decoction significantly reduced after treatment; liver cancer survival group mice showed significant intestinal, cecal, liver damage (mainly hyperplasia), slightly reduced brain mass, liver cancer, liver spleen deficiency group more obvious survival of mice more than Xiao Chai Hu Tang after treatment Significantly improved. Correlation analysis showed that there was a significant positive / negative correlation between small intestine damage, cecal damage, fecal accumulation of intestine, liver damage and brain mass in dead and surviving mice. Conclusion: Xiaochaihu decoction can reduce carbon tetrachloride by reducing intestinal - liver - brain injury, improving intestine - liver - brain interaction, protecting liver - spleen - qi deficiency and carbon tetrachloride / ethanol - induced intestinal - liver - brain injury and inflammation / Ethanol-induced liver cancer in mice when the mortality and the incidence of liver cancer, the molecular mechanism needs further elucidation.