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为探讨c-myb和bcl-2基因在C6脑胶质瘤细胞中的表达及其作用,作者采用c－myb反义硫代寡核苷酸（AON）进行裸鼠的体内试验。在裸鼠接种C6脑胶质瘤细胞株后10天，随机将动物分成正义治疗组(c－myb SON组)、反义治疗组(c－myb AON组)和对照组，每组12只。分别于治疗后第4、8、12、16天各处死动物3只，切取脑胶质瘤标本，用S－P免疫组化法观察c－myb和bcl－2蛋白的表达情况。结果：c－myb AON组c－myb蛋白和bcl－2蛋白阳性表达率与c－myb SON组和对照组比较，均有显著性差异（P<0.05）。从而提示c－myb蛋白和bcl－2蛋白可能与C6脑胶质瘤的发生、发展有关。
To investigate the expression and role of c-myb and bcl-2 genes in C6 glioma cells, the authors used c-myb antisense oligodeoxynucleotide (AON) to perform nude mice in vivo experiments. Ten days after nude mice were inoculated with C6 glioma cell line, the animals were randomly divided into a sense treatment group (c-myb SON group), an antisense treatment group (c-myb AON group) and a control group, 12 mice in each group. Animals were sacrificed on the 4th, 8th, 12th, and 16th day after treatment respectively. The glioma specimens were harvested and the expression of c-myb and bcl-2 proteins was observed by S-P immunohistochemistry. Results: The positive rates of c-myb protein and bcl-2 protein in c-myb AON group were significantly different from those in c-myb SON group and control group (P<0.05). This suggests that c-myb protein and bcl-2 protein may be related to the occurrence and development of C6 glioma.