目的建立凉粉草特征指纹图谱,为凉粉草的质量控制提供依据。方法采用HPLC法测定,色谱柱为DikmaDiamonsi(l 2)C18柱(250 mm×4.6 mm,5μm);流动相为乙腈-2%甲酸,梯度洗脱;检测波长为320 nm;体积流量为0.8 mL/min;柱温为25℃。结果建立了凉粉草的特征指纹图谱,34批次凉粉草确定10个共有峰。结论该法准确可靠,重复性好,为凉粉草质量控制提供了依据。 Objective To establish the fingerprint of jelly grass and provide the basis for the quality control of jelly grass. The method was performed on a Dikma Diamonsi (12) C18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile-2% formic acid by gradient elution. The detection wavelength was 320 nm and the volume flow rate was 0.8 mL / min; column temperature is 25 ℃. Results The fingerprint of jelly grass was established and 34 batches of jelly grass were identified as 10 common peaks. Conclusion The method is accurate and reliable with good repeatability and provides the basis for the quality control of jelly grass.