论文部分内容阅读
目的:研究上、下颌突间充质细胞在体外的成骨能力。方法 :体外培养胚胎13.5d(E13.5)的小鼠上、下颌突间充质细胞,观察其细胞形态。取第1代细胞进行成骨诱导培养7 d,通过免疫荧光、ALP染色、茜素红染色和qPCR检测其成骨能力。应用SPSS 18.0软件包对实验结果进行独立样本t检验。结果:E13.5的小鼠上、下颌突间充质细胞能在体外成功培养和传代。成骨诱导可促进上、下颌突间充质细胞表达成骨标志物,但下颌突间充质细胞成骨标志物OCN和OPN的表达、ALP染色和茜素红染色较上颌突间充质细胞弱。结论:上、下颌突间充质细胞在体外能成骨分化,下颌突间充质细胞较上颌突间充质细胞的成骨能力稍弱。
Objective: To study the osteogenic ability of upper and lower jaw mesenchymal cells in vitro. METHODS: Mesenchymal cells of the upper and lower jaws of embryonic 13.5d (E13.5) mice were cultured in vitro and their cell morphology was observed. The first generation cells were cultured for 7 days, and their osteogenic ability was detected by immunofluorescence, ALP staining, alizarin red staining and qPCR. SPSS 18.0 software package was used to test the results of independent samples t-test. RESULTS: Upper and lower jaw mesenchymal cells in E13.5 mice were successfully cultured and passaged in vitro. Osteogenetic induction promoted the expression of osteogenic markers in mesenchymal cells in both maxillary and mandibular formations. However, OCN and OPN expression in osteoblasts of mandibular mesenchymal cells were significantly higher than those in maxillary mesenchymal cells weak. Conclusion: The mesenchymal cells of the upper and lower jaws can differentiate into osteoblasts in vitro, and the mesenchymal cells of the mandibular process are weaker than the osteogenic capacity of the maxillary prominence mesenchymal cells.