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目的观察慢性HBV感染孕妇外周血树突状细胞(DCs)的表型及功能。方法对20例慢性HBV感染孕妇(A组)外周血来源DCs进行体外培养,应用流式细胞术检测DCs的表面分子HLA-DR、CD80、CD86的表达,并与15例健康孕妇(B组)及15例非孕HBV感染育龄妇女(C组)对比,分析DCs在慢性HBV感染孕妇中的功能特点。结果 A组DCs体外培养细胞数量低于B组(P<0.05),比C组略高,但无统计学差异(P>0.05)。A组DCs表面分子HLA-DR、CD80、CD86的表达均低于B组(P<0.05),比C组数值均略高,也无统计学差异(P>0.05)。结论慢性HBV感染孕妇与非孕HBV感染育龄妇女一样存在着DCs表型和功能的缺失,但可能由于体内免疫机制的改变,DCs表型和功能较非孕HBV感染育龄妇女有增强的趋势,需进一步扩大研究。
Objective To observe the phenotype and function of peripheral blood dendritic cells (DCs) in pregnant women with chronic HBV infection. Methods Twenty DCs from peripheral blood of 20 pregnant women with chronic HBV infection (group A) were cultured in vitro. The expression of HLA-DR, CD80 and CD86 on DCs was detected by flow cytometry and compared with 15 healthy pregnant women (group B) And 15 non-pregnant women infected with HBV infection (group C). The functional characteristics of DCs in pregnant women with chronic HBV infection were analyzed. Results The number of DCs in group A was lower than that in group B (P <0.05), which was slightly higher than that in group C (P> 0.05). The expressions of HLA-DR, CD80 and CD86 on the surface of DCs in group A were all lower than those in group B (P <0.05), which were slightly higher than those in group C (P> 0.05). Conclusion There is a lack of phenotype and function of DCs in pregnant women with chronic HBV infection as women of childbearing age who are not infected with HBV. However, the phenotypes and functions of DCs tend to be stronger than that of women of childbearing age who are not infected with HBV due to the change of immune mechanisms in vivo. Further expand the research.