论文部分内容阅读
目的探讨乙肝病毒侵犯淋巴细胞对慢性乙肝患者免疫功能状态的影响。方法采用双抗体夹心法检测慢性乙型肝炎患者外周血单个核细胞（ＰＢＭＣ）培养上清液中细胞因子的水平， ＰＣＲ方法扩增乙肝病毒ＤＮＡ片段。血清中ＨＢＶ ＤＮＡ检测采用斑点杂交法。结果与正常对照组比较，慢性乙型肝炎患者的 ＩＦＮ－γ水平降低，但无显著差异性：而ＩＬ－４水平明显增高（Ｆ＝１７.４，Ｐ＜０．０１）。血清中ＨＢＶ ＤＮＡ水平与 ＰＢＭＣ培养上清液中的ＩＦＮ－γ水平呈负相关（ｒ_ｓ＝－０.５３，Ｐ＜０.０１）。６２.９％（３５／３６）的慢性乙肝患者外周血ＰＢＭＣ中检出ＨＢＶ ＤＮＡ，ＨＢＶ ＤＮＡ阳性组和阴性组的ＩＦＮ－γ水平具有显著差异（Ｆ＝１５.２，Ｐ＜０.０１）。结论（１）慢性乙肝患者的外周血以 Ｔｈ_２类细胞因子占优势。（２）乙肝病毒侵犯淋巴细胞可能影响其免疫功能的发挥。
Objective To investigate the effect of hepatitis B virus-infected lymphocytes on immune function in chronic hepatitis B patients. Methods The levels of cytokines in peripheral blood mononuclear cells (PBMCs) from patients with chronic hepatitis B were detected by double antibody sandwich method. The HBV DNA fragments were amplified by PCR. Serum HBV DNA detection using dot blot hybridization. Results Compared with the normal control group, IFN-γlevels in patients with chronic hepatitis B were decreased, but there was no significant difference: IL-4 level was significantly higher (F = 17.4, P <0.01). There was a negative correlation between serum HBV DNA level and IFN-γ level in PBMC culture supernatant (r_s = -0.53, P <0.01). HBV DNA was detected in peripheral blood of 62.9% (35/36) patients with chronic hepatitis B, IFN-γ levels of HBV DNA positive and negative groups were significantly different (F = 15.2, P <0.01) . Conclusion (1) Th2 type cytokines predominate in the peripheral blood of patients with chronic hepatitis B (2) Hepatitis B virus invasion of lymphocytes may affect their immune function.