对兔晶体上皮细胞培养方法作了改进,为探讨后发性白内障的预防提供实验手段。取新西兰家兔晶体前囊膜作细胞培养。在原代培养中,以吸管转移囊膜。细胞融合后,用胰酶消化转代。结果:原代培养48～72小时后,可见晶体上皮细胞长出,以后细胞呈贴壁单层,铺砌型向外生长。传代后6～8小时细胞贴壁生长。方法经济简便,可为各种实验提供兔晶体上皮细胞。 The methods of culturing rabbit lens epithelial cells were improved, which provided experimental means for the prevention of posterior capsular disease. Take New Zealand rabbit pre-lens capsule for cell culture. In primary culture, the capsule is transferred by pipette. After the cells have been fused, they are digested with trypsin for substitution. RESULTS: After 48-72 hours of primary culture, the crystal epithelial cells were seen to grow. Afterwards, the cells were adherent monolayer and the pavement type was outward growth. 6 to 8 hours after passage adherent cells grow. The method is simple and economical and can provide rabbit lens epithelial cells for various experiments.