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探讨硒对大鼠大脑皮层神经元存活及突起生长的影响 ,利用新生 SD大鼠大脑皮层神经元体外分散培养技术 ,培养液中加入不同浓度的硒 ( 6 2 .5 μg/ L,12 5 .0 μg/ L,182 .5 μg/ L)并以神经生长因子 ( NGF)作阳性对照 ,测量了 16 h、2 4h、36 h、48h小时后神经元最长突起长度及平均每视野神经元存活数 ( 3d,5 d,7d)。结果显示 ,低浓度的硒与空白对照组相比可见加硒组存活数增加 ,突起生长早且长。低浓度硒对大脑皮层神经元存活及突起生长有促进作用
To investigate the effects of selenium on neuronal survival and neurite outgrowth in rat cerebral cortex. Separation and culture of neurons in neonatal SD rat cortical neurons were performed in vitro. Different concentrations of selenium (62.5 μg / L, 125) were added to the culture medium. 0 μg / L, 182 .5 μg / L) and nerve growth factor (NGF) as a positive control, measured at 16 h, 2 4 h, 36 h, 48 h after the longest neurite projection length and average per field neurons Survival number (3d, 5d, 7d). The results showed that low concentrations of selenium compared with the blank control group can be seen increased survival of selenium group, the protuberance grows early and long. Low concentration of selenium can promote neuronal survival and neurite outgrowth in cerebral cortex