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5-甲基胞嘧啶在哺乳动物细胞中具有广泛的作用.而它被双脱氧家族Tet蛋白氧化所得的产物5-羟甲基胞嘧啶、5-醛基胞嘧啶和5-羧基胞嘧啶也被证明在细胞发育和5-甲基胞嘧啶动态平衡调控中具有关键的作用.已有的研究结果表明,Tet蛋白能够识别双链DNA上的5-甲基胞嘧啶,并将其氧化成5-羟甲基胞嘧啶、5-醛基胞嘧啶和5-羧基胞嘧啶.我们通过质谱仪检测发现,老鼠Tet1蛋白的DNA结合结构域还能识别和氧化单链DNA上的5-甲基胞嘧啶.这一发现暗示我们,Tet蛋白家族不但具有已经发现的氧化双链DNA上5-甲基胞嘧啶的功能,还有可能作用于DNA的复制及转录,甚至具有氧化单链RNA上对应的甲基修饰碱基的能力.
5-Methylcytosine has a wide range of effects in mammalian cells, and its products 5-hydroxymethylcytosine, 5-aldehyde cytosine and 5-carboxycytosine, which are oxidized by the dideoxy family Tet protein, have also been Proved to be crucial in cell development and regulation of 5-methylcytosine homeostasis.Existing studies have shown that the Tet protein recognizes 5-methylcytosine on double-stranded DNA and oxidizes it to 5- Hydroxymethylcytosine, 5-aldehyde cytosine and 5-carboxycytosine.We detected by mass spectrometry found that the DNA binding domain of rat Tet1 protein can also recognize and oxidize 5-methylcytosine on single-stranded DNA This finding suggests that our family of Tet proteins not only have been found to function as 5-methylcytosine on oxidized double-stranded DNA, but may also act on DNA replication and transcription, and even have a counterpart on oxidized single-stranded RNA The ability of a base to modify a base.