In order to observe several antibodies to liver antigens in Chinese patients with different liver diseases and to discuss the characteristics of the autoantibodies in autoimmune liver diseases, from 1412 patients, detected by indirect immunofluorescence (IIF) initially, 230 patients with abnormal ALT were chosen and divided into 5 groups: ① autoimmune diseases group, 42 cases: 18 with autoimmune hepatitis (AIH), 21 with primary biliary cirrhosis (PBC), 3 with primary sclerosing cholangitis(PSC). ② HAV group, 23 cases; ③ HBV group, 70 cases; ④ HCV group, 35 cases and ⑤ Non A-E group, 60 cases. First, ANA, AMA, SMA, liver-kidney microsomal antibody (LKM) and so on were tested by IIF.Then, LKM-1, liver cytosolic-1 (LC-1), soluble liver antigen/liver pancreas (SLA/LP) and subtype of AMA (M2) as well as ANA profile such as SS-A, SS-B and dsDNA were tested by Western blot and immunoblot strips assay, respectively. The results were that among 1412 cases, those diagnosed as AIH, PBC and PSC accounted for 12.7‰, 14.9‰ and 2.1‰, respectively, of the samples being tested. 2/230 with LKM-1 and 2/230 with SLA/LP were seen in individuals infected with AIH and HCV, respectively. All patients with PBC showed AMA and M2 antibodies. No specific ANA pattern was seen in AIH by IIF but anti-actin was only found in patients with AIH. In Non A-E group, four cases were positive of AMA and M2; three had high titer of SMA and other 4 had SS-A, SS-B or dsDNA antibodies, etc. It was concluded that the detection of anti-liver antigens, ANA profile and AMA subtypes were helpful for the diagnosis of autoimmune liver diseases and overlap syndromes. In patients with Non A-E hepatitis, the diagnosis of PBC or AIH should be taken into consideration. In order to observe several antibodies to liver antigens in Chinese patients with different liver diseases and to discuss the characteristics of the autoantibodies in autoimmune liver diseases, from 1412 patients, detected by indirect immunofluorescence (IIF) initially, 230 patients with abnormal ALT were chosen and HCC group, 23 cases; ③ HBV group, 23 cases with autoimmune hepatitis (AIH), 21 with primary biliary cirrhosis (PBC), 3 with primary sclerosing cholangitis First ANA, AMA, SMA, liver-kidney microsomal antibody (LKM) and so on were tested by IIF. Then, LKM-1, liver cytosolic (LC-1), soluble liver antigen / liver pancreas (SLA / LP) and subtype of AMA (M2) as well as ANA profile such as SS-A, SS- B and dsDNA were tested by Western blot and immunoblot strips assay, respectively. The results were that among among 1412 cases, those diagnosed as AIH, PBC and PSC accounted for 12.7 ‰, 14.9 ‰ and 2.1 ‰, respectively, of the samples being tested. 2/230 with LKM-1 and 2/230 with SLA / LP were seen in individuals infected with AIH and HCV, respectively. All patients with PBC showed AMA and M2 antibodies. No specific ANA pattern was seen in AIH by IIF but anti-actin was only found in patients with AIH. In Non AE group, four cases were positive of AMA and M2; three had high titer of SMA and other 4 had SS-A, SS-B or dsDNA antibodies, etc. It was concluded that the detection of anti-liver antigens, ANA profile and AMA subtypes were helpful for the diagnosis of autoimmune liver diseases and overlap syndromes. In patients with Non AE hepatitis, the diagnosis of PBC or AIH should be taken into consideration.