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目的:观察布洛芬对甲醛刺激大鼠足底引发脊髓神经元Fos蛋白表达的影响,探讨布洛芬对伤害性刺激的抑制作用。方法:SD大鼠30只,随机分为空白对照刺激组(A组),生理盐水注射液组(对照组B组),布洛芬低剂量组(C1组),布洛芬中剂量组(C2组),布洛芬高剂量组(C3组),每组6只。甲醛刺激180min后,用行为学机械触诱发痛阈值测定和免疫组织化学染色法分析大鼠脊髓神经元Fos蛋白表达量及分布状况。结果:30只大鼠均进入结果分析,显示:C1,C2和C3组的机械触诱发痛阈值较对照A组和B组均明显升高(P<0.05),C3组明显高于C1和C2组(P<0.05);而后分析受甲醛刺激后180min时的情况:A组和B组见脊髓背角浅层有大量淡染Fos样免疫阳性神经元,其中A,B均明显多于C1,C2和C3组(P<0.05)。C3组Fos样免疫阳性神经元数明显少于C1和C2组(P<0.05)。结论:布洛芬处理后能对伤害性刺激引起的大鼠机械触诱发痛阈值部分升高,处理能够对脊髓背角浅层Fos样免疫阳性神经元增多效应产生抑制作用;布洛芬可以抑制大鼠Fos样免疫阳性神经元产生,这种表现可能与布洛芬抑制体内前列腺素E合成有关。
Objective: To observe the effect of ibuprofen on the expression of Fos protein in spinal cord induced by formaldehyde in rat soleus and the inhibitory effect of ibuprofen on noxious stimulation. Methods: Thirty SD rats were randomly divided into blank control group (group A), saline injection group (control group B), ibuprofen low dose group (group C1), ibuprofen middle dose group C2 group), high-dose ibuprofen group (C3 group), 6 in each group. After 180 minutes of formalin stimulation, the expression and distribution of Fos protein in rat spinal cord neurons were analyzed by behavioral mechanical tactile allodynia thresholds and immunohistochemical staining. Results: Thirty rats were involved in the analysis of the results. The results showed that the thresholds of mechanical allodynia in C1, C2 and C3 groups were significantly higher than those in control A and B groups (P <0.05), C3 group was significantly higher than C1 and C2 (P <0.05) .After 180min stimulation with formaldehyde, group A and group B showed a large number of Fos-like immunoreactive neurons in the superficial layers of spinal dorsal horn, in which A and B were significantly more than those of C1, C2 and C3 (P <0.05). The number of Fos-like immunoreactive neurons in C3 group was significantly less than that in C1 and C2 groups (P <0.05). Conclusion: After ibuprofen treatment, the threshold of mechanical tachyphrytic pain induced by noxious stimulation is partly increased, and the treatment can inhibit the increase of Fos-like immunoreactive neurons in the superficial dorsal horn of spinal cord. Ibuprofen can inhibit Fos-like immunopositive neurons in rat are produced, which may be related to the inhibition of prostaglandin E synthesis by ibuprofen in vivo.